Here is an update-
the procedure went perfectly. I used a brand new sterile razor blade, and I basically found almost no blood from the stump. I placed him back in a container of new water, with the melafix in it. I will be keeping him cool for the next few days, but I am wondering still about the dosedge on the melafix, and how low the temp should be, as I do want him to heal and that means blood flow and other considerations with temperature.