The longest running Amphibian Community on the Internet.

Tags Register FAQ Calendar Search Today's Posts Mark Forums Read Caudata.org Store


Daphnia aeration in 2 liter bottles?

This is a discussion on Daphnia aeration in 2 liter bottles? within the Scuds, Freshwater Shrimp, Slaters, Woodlice, etc forums, part of the Food: Live, Frozen, Freeze-Dried, Pellets, etc category; I've recently started culturing daphnia again. I have 2 cultures in 2-liter soda bottles and feed them active yeast and ...

Like Tree1Likes
  • 1 Post By oceanblue

Reply

 

LinkBack Thread Tools Display Modes
Old 3rd August 2013   #1 (permalink)
Member
 
Join Date: Apr 2009
Nationality:
Posts: 252
Gallery Images: 0
Comments: 1
Rep: stanleyc has given good advice and informationstanleyc has given good advice and information
Default Daphnia aeration in 2 liter bottles?

I've recently started culturing daphnia again. I have 2 cultures in 2-liter soda bottles and feed them active yeast and spirulina powder. I've used the same setup and foods in the past and had good enough production for the most part. But feeding them yeast and spirulina, there's always a chance of fouling the water, which has cost me a couple times in the past.

I was wondering if aeration is worth doing in such small containers. I've tried an air pump/tubing setup, but it produces too strong a current in the 2-liter bottles. Is there a way to make this setup work? Is there something else that would work in such small containers? Or should I not bother with aeration and just be careful with feeding and maintenance?

Thanks



stanleyc is offline   Reply With Quote
Old 3rd August 2013   #2 (permalink)
Prolific Member
 
Join Date: Sep 2007
Nationality:
Location: [ Members Only ]
Age: 65
Posts: 654
Gallery Images: 1
Comments: 3
Rep: oceanblue goes to 11oceanblue goes to 11oceanblue goes to 11oceanblue goes to 11oceanblue goes to 11oceanblue goes to 11oceanblue goes to 11oceanblue goes to 11oceanblue goes to 11oceanblue goes to 11oceanblue goes to 11
Default Re: Daphnia aeration in 2 liter bottles?

I've not found 2L pop bottles very good for daphnia, but even smaller flat rectangular 650ml plastic containers, only filled to 400ml, from the local take-away restaurant are very good at maintaining small back up cultures without aeration, and I've even kept a few daphnia in a petri dish stack.

The containers are a lot easier to pipette out or net a few daphnia than bottles which have to be decanted to get at the contents, or if you scissor off the top they become floppy and hard to handle.

Shallow seems to work very well without aeration, I presume diffusion works well with the wide surface area. You could stick with 2L pop bottles but only put 800ml in them and stack them sideways like wine.



oceanblue is offline   Reply With Quote
Old 3rd August 2013   #3 (permalink)
Administrator
 
Jennewt's Avatar
 
Join Date: May 2005
Nationality:
Location: [ Members Only ]
Age: 54
Posts: 12,378
Gallery Images: 97
Comments: 46
Rep: Jennewt goes to 11Jennewt goes to 11Jennewt goes to 11Jennewt goes to 11Jennewt goes to 11Jennewt goes to 11Jennewt goes to 11Jennewt goes to 11Jennewt goes to 11Jennewt goes to 11Jennewt goes to 11
Default Re: Daphnia aeration in 2 liter bottles?

I would recommend using larger containers, if possible. The problem of too strong aeration can be solved with an air control kit (little plastic splitters and valves). Put this search into google: "aquarium air tubing control kit".



__________________
Useful Links: Caresheets | Newt & Salamander FAQs | Axolotl FAQs |My website | Forum Rules.
Jennewt is offline   Reply With Quote
Old 3rd August 2013   #4 (permalink)
Prolific Member
 
Coastal Groovin's Avatar
 
Join Date: Nov 2008
Nationality:
Location: [ Members Only ]
Posts: 923
Gallery Images: 1
Comments: 7
Rep: Coastal Groovin is a mainstay of Caudata.orgCoastal Groovin is a mainstay of Caudata.orgCoastal Groovin is a mainstay of Caudata.orgCoastal Groovin is a mainstay of Caudata.orgCoastal Groovin is a mainstay of Caudata.orgCoastal Groovin is a mainstay of Caudata.orgCoastal Groovin is a mainstay of Caudata.orgCoastal Groovin is a mainstay of Caudata.org
Default Re: Daphnia aeration in 2 liter bottles?

Aeration normally kills daphnia. Banging them into each other and the walls of the container. This grinds away their shells. I would try to use 5-10 gallons tanks and feed green water. Culture green water from turtle tanks or grown in large soda bottles filled with rain water and liquid fertilizer set to grow on a sunny windowsill. If you don't want to use green water try crushed up goldfish flakes.



__________________
Axolotls the other white meat.
Coastal Groovin is offline   Reply With Quote
Old 3rd August 2013   #5 (permalink)
Member
 
Join Date: Apr 2009
Nationality:
Posts: 252
Gallery Images: 0
Comments: 1
Rep: stanleyc has given good advice and informationstanleyc has given good advice and information
Default Re: Daphnia aeration in 2 liter bottles?

With the space I have available, I think I'll stick with the bottles for now. I will try to the tubing control kit.

Thanks for the advice both of you.



stanleyc is offline   Reply With Quote
Old 8th October 2013   #6 (permalink)
Junior Member
 
Join Date: Sep 2013
Nationality:
Location: [ Members Only ]
Posts: 13
Gallery Images: 0
Comments: 0
Rep: mjmpt is an unknown quantity at this point
Default Re: Daphnia aeration in 2 liter bottles?

I use 2 l bottles, full up to about 5cm from the top, without aeration. But I use almost exclusivelly greenwater and tank water. When I do have to feed something else, I use only 2-3 drops per bottle, of bakers yeast, liquizell or a similar suspension I prepare.



mjmpt is offline   Reply With Quote
Reply

LinkBack
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On


Similar Threads

Thread Thread Starter Forum Replies Last Post
Upgrading 60 liter (15gal) to 200 liter (55 gal) tank and need some advice Sherleelee Axolotl tank set-ups, filters, substrate 1 11th July 2013 00:57
Frozen bottles/ice packs/ice cubes. Erinony Newt and Salamander Help 2 18th June 2013 13:43
How many 5 inch newts (N. v.) could I fit in a 125 gallon (475 liter) tank? sharrakor Newt and Salamander Help 5 14th September 2012 12:16
How many axolotls can live in 102 liter tank wian Axolotl General Discussion 4 23rd April 2012 00:13
Cycling new tanks / Bacteria in bottles FRANCOIS Newt and Salamander Help 1 20th August 2011 21:38


All times are GMT. The time now is 12:45.