The longest running Amphibian Community on the Internet.

Tags Register FAQ Calendar Search Today's Posts Mark Forums Read Caudata.org Store


Skin secretion sampling methodology?

This is a discussion on Skin secretion sampling methodology? within the Other Scientific Studies forums, part of the Herpetological Science & Politics category; Anyone familiar with dermal secretion sampling techniques for newts? I suspect swabs may be the right way, but I wonder ...

Other Scientific Studies For discussion of scientific studies not covered under other "science & politics" topics.

Like Tree1Likes
  • 1 Post By JacksonR

Reply

 

LinkBack Thread Tools Display Modes
Old 29th November 2011   #1 (permalink)
Site Contributor
 
SludgeMunkey's Avatar
 
Join Date: Nov 2008
Nationality:
Location: [ Members Only ]
Age: 41
Posts: 2,299
Gallery Images: 42
Comments: 9
Rep: SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11
Exclamation Skin secretion sampling methodology?

Anyone familiar with dermal secretion sampling techniques for newts?

I suspect swabs may be the right way, but I wonder if a small tissue sample is also necessitous.

My thesis finally was approved today and I now have access to a entire range of testing I have never had before.

(Without going into too much detail at this time, The results of this project will be a comprehensive analysis of an under-documented species.)

I am having difficulty finding concrete instruction on the procedure.

If there are publications you could direct me to I would be forever grateful. To date I have had no luck in finding caudate specific methods. I suspect that given the nature of this species undocumented toxin(s), dermal swabs as used with certain frogs may not provide reliable samples.

In short, I am trying to save money by "getting it right the first time".


As always, any or all advice is appreciated.



SludgeMunkey is offline   Reply With Quote
Old 29th November 2011   #2 (permalink)
Banned
 
Join Date: Mar 2011
Nationality:
Posts: 72
Gallery Images: 0
Comments: 0
Rep: JacksonR has started on the right path
Default Re: Skin secretion sampling methodology?

http://frogchemistry.com/PDFs/ClarkV...20analyses.pdf


This might help.



JacksonR is offline   Reply With Quote
Old 30th November 2011   #3 (permalink)
Site Contributor
 
SludgeMunkey's Avatar
 
Join Date: Nov 2008
Nationality:
Location: [ Members Only ]
Age: 41
Posts: 2,299
Gallery Images: 42
Comments: 9
Rep: SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11
Default Re: Skin secretion sampling methodology?

BINGO!


Thank you!

This is exactly the sort of thing I have been trying to find for weeks now. (Actually, I'm shocked I didn't with all the digging I have been doing!)

Thank you! Thank you! Thank you!




Last edited by SludgeMunkey; 30th November 2011 at 02:18.
SludgeMunkey is offline   Reply With Quote
Old 30th November 2011   #4 (permalink)
Site Contributor
 
SludgeMunkey's Avatar
 
Join Date: Nov 2008
Nationality:
Location: [ Members Only ]
Age: 41
Posts: 2,299
Gallery Images: 42
Comments: 9
Rep: SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11
Default Re: Skin secretion sampling methodology?

I probably sound like the amateur turned undergrad that I am, but if anyone has any interest in this sort of research, I suggest you read the paper Jackson provided.

Sometimes one can't see the forest for the trees, and in this case a fresh set of eyes did the trick.



SludgeMunkey is offline   Reply With Quote
Old 30th November 2011   #5 (permalink)
Banned
 
Join Date: Mar 2011
Nationality:
Posts: 72
Gallery Images: 0
Comments: 0
Rep: JacksonR has started on the right path
Default Re: Skin secretion sampling methodology?

I'm flattered. lol But really i'm the amature here! Happy to help.

And I think I know the species you're going to study.



JacksonR is offline   Reply With Quote
Old 1st December 2011   #6 (permalink)
Site Contributor
 
SludgeMunkey's Avatar
 
Join Date: Nov 2008
Nationality:
Location: [ Members Only ]
Age: 41
Posts: 2,299
Gallery Images: 42
Comments: 9
Rep: SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11SludgeMunkey goes to 11
Default Re: Skin secretion sampling methodology?

Aye, it is obvious isn't it?


The good news is I have all sorts of testing lined up to analyze the toxin.

I am waiting for a few items to show up in the mail so I can start harvesting and prepping samples for Raman, FTIR, chromatography and spectrography.

I'm excited to find out what this stuff is as I can speak from experience, it makes me sicker than a dog very quickly, just by smelling it.



SludgeMunkey is offline   Reply With Quote
Old 1st December 2011   #7 (permalink)
Prolific Member
 
Linus's Avatar
 
Join Date: Dec 2009
Nationality:
Location: [ Members Only ]
Posts: 506
Gallery Images: 0
Comments: 3
Rep: Linus is a well respected, valued and knowledgeable member of Caudata.orgLinus is a well respected, valued and knowledgeable member of Caudata.orgLinus is a well respected, valued and knowledgeable member of Caudata.orgLinus is a well respected, valued and knowledgeable member of Caudata.orgLinus is a well respected, valued and knowledgeable member of Caudata.orgLinus is a well respected, valued and knowledgeable member of Caudata.org
Default Re: Skin secretion sampling methodology?

Very interested to hear the results!



Linus is offline   Reply With Quote
Reply

LinkBack
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On


Similar Threads

Thread Thread Starter Forum Replies Last Post
Illness/Sickness: Skin peeling sophieaxolotl Sick Axolotl? 2 28th October 2009 14:23
Skin shedding or skin disease? Maldark Sick Axolotl? 14 29th April 2009 13:37
Flaky Skin??? brendan Sick Axolotl? 3 5th February 2005 07:40
Flaking skin shelagh Sick Axolotl? 2 10th January 2005 22:04
Losing skin katy Sick Axolotl? 6 27th September 2004 14:40


All times are GMT. The time now is 22:07.