Photos of jeffersons
This is a discussion on Photos of jeffersons within the Mole Salamanders but not tigers or axolotls (Ambystomatids) forums, part of the Species, Genus & Family Discussions category; i got a break in school-test is moved to next week so i decided to post some pics i was ...
|Mole Salamanders but not tigers or axolotls (Ambystomatids) These large-mouthed, burrowing salamanders are indigenous to Central and North America.|
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|31st March 2004||#1 (permalink)|
i got a break in school-test is moved to next week so i decided to post some pics i was promising. these arent too outstanding cause i was limited by light, plus these and the giants are pretty shiny sals and i have to try hard to reduce glare-i thinking one of those light umbrellas are probably in my future too. these were raised from an egg mass collected by a teacher and are about 4 years old now.by next semester i will be able for sure to id them as either true jeffersons or as jefferson complex ones-i will be taking genetics lab and the teacher already said i can modify the course so i can do sal DNA and not plants and dog testicles
i am hoping that i can get enough dna from a toe clipping and some amplification techniques.
ok here are some head shots
the pattern is hard to see but its a slate-ish gray body with light bluish snowflake marks on it. here is one of the only full body shots that came out ok.
and finally one obligatory ultra close head shot
|7th October 2004||#6 (permalink)|
i don't know about DNA from shed skin in salamanders... i would guess you wouldn't get very much but i don't know for sure.
i have done some genetic work with newt tissues (Taricha, and taking a bit of the tail worked quite well. it didn't make them bleed and it regenerated quickly. we then chop up the tissue and use a kit to extract the DNA from it. then use those extracts for PCR. its not uncommon to have so much DNA in the extract that it has to be diluted before using it for PCR.
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