good question, ph does fluctuate a bit depending on the time of day i think and also if the water is fresh out of the tap too, i dont know why exactly but it normally drops within the next 24 hours or so. it also depends on where you get the water from in the tank too, top or bottom etc, youd think ph would be straight foward aye hehe. im not sure why the different levels make different ph also sorry.
so you left it over night and it was (what colour) then changed to green after? a grass green is normally around neutral, a yellowy green is more acidic and alkaline is a bluey green (then you get to the extremes like purple and stuff). i assume leaving the chemicals in the tube so long would react different to a fresh test, maybe the contact with air etc?