Bacillus thuringiensis

eyrops

New member
Joined
Dec 19, 2007
Messages
104
Reaction score
12
Points
0
Location
Morrisville, Pennsylvania
Country
United States
Display Name
Steve Morse
I have been interested in using [FONT=&quot]Bacillus thuringiensis [/FONT][FONT=&quot]in an outdoor amphibian setup to kill mosquito larvae. Mosquitoes have become more than a nuisance issue in many portions of the US because they have the potential to be carriers of West Nile virus, which can be serious and occasionally fatal for humans. I recently read of a New York City resident who was fined about $300 for operating a birdbath. While this incident is certainly not typical today in most places, the mentality that created it is on the rise. I believe that controlling mosquitoes in some manner is a responsibility of a hobbyist setting up an outdoor amphibian enclosure. One method would be to stock an organism that would eat the mosquito larvae. This may be a viable approach if the right organism for the particular setup can be found. I am not discussing this approach here. A second approach could be to use a pesticide that targets mosquito[/FONT][FONT=&quot]es without being detrimental to the amphibians. One possibility for this is the bacteria[/FONT][FONT=&quot]Bacillus thuringiensis[/FONT][FONT=&quot]. [/FONT][FONT=&quot]These bacteria can be deadly to mosquitoes and many other insects, and is commonly claimed to be allowable under the “organic” gardening and farming umbrella. But what effect do they have on amphibians? I had not found very much information on Caudata.org or elsewhere. I decided to do a very simple qualitative trial with two [/FONT][FONT=&quot]Rana catesbeiana[/FONT][FONT=&quot]tadpoles.[/FONT]

[FONT=&quot]I put each in a bucket with about 1 ½ gallons of water. I added a commercia[/FONT][FONT=&quot]l[/FONT][FONT=&quot]Bacillus thuringiensis[/FONT][FONT=&quot]product to one at a visual approximation of the recommended rate. I added some [/FONT][FONT=&quot]Daphnia[/FONT][FONT=&quot] to both buckets. Five days later, both tadpoles are alive, and both buckets contained living [/FONT][FONT=&quot]Daphnia[/FONT][FONT=&quot]. [/FONT][FONT=&quot] Concurrently, I set up two pint jars with mosquito larvae, on with[/FONT][FONT=&quot]Bacillus thuringiensis[/FONT][FONT=&quot], a[/FONT][FONT=&quot]nd one without. The mosquito larva in the jar with the[/FONT][FONT=&quot]Bacillus thuringiensis [/FONT][FONT=&quot]died within 24 hours. There were still a few pupae alive. These were not evident after 48 hours. The larvae in the untreated jar appeared fine.[/FONT]

[FONT=&quot]This was a simple qualitative trial, not a quantitative experiment with replicates. It proves nothing except that[/FONT][FONT=&quot]Bacillus thuringiensis[/FONT][FONT=&quot]is not universally fatal to [/FONT][FONT=&quot]Rana catesbeiana[/FONT][FONT=&quot]tadpoles over the short term. I think it does suggest possibilities for more formal experiments. Although it seems cold and calculating, it also seems to me to be work that needs to be done. I hope to learn the experiences of others, and perhaps see some links to scientific papers on the topic posted here.[/FONT]
 

Attachments

  • DSCN7462adjSML.jpg
    DSCN7462adjSML.jpg
    323.8 KB · Views: 1,351
  • DSCN7452adjSML.jpg
    DSCN7452adjSML.jpg
    258 KB · Views: 1,313
  • DSCN7457adjSML.jpg
    DSCN7457adjSML.jpg
    310.8 KB · Views: 2,084
General chit-chat
Help Users
  • No one is chatting at the moment.
  • Robpod86:
    Hi everyone. I've had several tiger salamanders in the past and never had this happened. My salamander has been bloated for 2 and a half weeks now. I looked it up and was pretty sad to see the info.
    +1
    Unlike
  • Robpod86:
    I posted prematurely, is there anything I can do to treat my little guy?
    +1
    Unlike
  • Ed69:
    This is regarding the California slender garden salamanders.
    +1
    Unlike
  • KirbyandYoshi:
    I had a axolotl hatch after 7 days of being layed and my brine shrimp won't be ready till Monday for feeding. Will he be able to make it or is there anything I can try feeding him in the meantime?
    +1
    Unlike
  • Jankowpa01:
    I do not have my Axolotl’s yet waiting for my cycle to complete! I am towards the end of my 30g fishless cycle. It has been a long journey. I have easily been processing 4ppm of ammonia in 24 hours for about a week.Each night I add 4ppm ammonia ( Dr Tims) and test every 12 hrs and nitrites read around 2ppm but when I test after 24hrs they are at 0 nitrites. I am using API master Kit. Should nitrites be constantly 0 in a 24 hour period or is a spike 12 hours later normal? Is my tank cycled? PH- is 7.4 Nitrates- 40ppm and will do water change once I am sure tank is cycled
    +1
    Unlike
  • SubZero:
    I'm not a Axolotl keeper but that sounds good
    +1
    Unlike
  • Paige1warren:
    Hi guys! I just have a quick question regarding axoltols. I’ve had my lotl since October and she is a year old now! Anyways, her water has been on the warmer side of the spectrum (about 69-70 degrees F, 20ish C). I finally got a fan to blow over the top of the tank, and it decreased to about 64 f in 2 hours. Super happy to be able to keep the water cooler, but I was wondering if axoltols have any reaction to the water suddenly becoming cold? Should I lower the temp more slowly? My lotl isn’t acting stressed or anything, but I just want to make sure there won’t be any problems. Thanks!
    +1
    Unlike
  • Paige1warren:
    Also, she was fine at the higher temperature. Perfectly healthy and never showed signs of de stress. Just thought it being colder would make her happier and digest a little slower! (She’s a pig :p )
    +1
    Unlike
  • SubZero:
    is it normal for a tiger to jump at you from being the glass
    +1
    Unlike
  • Murk:
    @Paige1warren That should be fine. I wouldn't suddenly put her in very cold water without acclimatisation, but a few degrees over the course of two hours shouldn't be a problem at all.
    +1
    Unlike
  • AlexisJG:
    Is anyone online? I need help badly
    +1
    Unlike
  • AlexisJG:
    I have had my 2 axolotls since June 2020 and I had hoped they would mature the same gender but unfortunately they are not. I am currently on vacation and planned to separate them once I returned, my axolotls are currently being cared for by a friend who knows the basics to care for them properly. A melanoid and a leucistic. I had a feeling but didn't prioritize it enough and now I have many many eggs in the tank with both of my axolotls. I have zero experience with eggs and cannot really care for juvenile axolotls but I really DO NOT want to kill them. What should I do? Are my axolotls okay being in the same tank with them? would the eggs be okay all together in a tank by themselves? Where can I find someone to care for them and hatch them?I'm so stressed and worried I just want the best for them all.
    +1
    Unlike
  • axie owner:
    you are going to have to get a bucket and put the eggs in that. they can hatch anytime between 11 and 20 days. order in brine shrimp eggs and get your friend to watch a video on hatching them. make sure your friend does 100% water changes every day. keep the water around 64digres and make sure your friend sends daily updates on them. i found kitchen salt works for the brine shrimp eggs. hope this helps.
    +1
    Unlike
  • Gots2knowme2:
    I am a new owner of two juvenile axolotls that are Lucie's. One has black eyes and one has silver eyes. Is that common or rare?
    +1
    Unlike
  • FP.Foxy:
    A few days ago, my Axolotl was about to die, there was something wrong with the water but he’s doing fine now. His skin is still cracked and his red things are still white
    +1
    Unlike
  • Paige1warren:
    Hi guys.. I might of made an error and need some advice. One of the hides in my tank is too small for my axie now, but I did not want to buy another hide because they are expensive, so I decided to DIY one. I used a Tupperware (cut into a little home) and glued rocks from outside and plants on it. The problem is, I washed the rocks with soap and hot water. I completely wasn’t thinking. When I washed them there was no soap residue that was visible on the rocks, and the rocks have been drying for 3 days on the container. So it has been 3 days since I washed them. I used aquarium safe silicone to glue them on. It was fully dry tonight so I decided to put it in my tank. I had it in for about 3 mins until I realized j had washed the rocks in soap, and I took it out immediately. Should I be worried??? I’m really freaking out, I don’t want anything to happen to my baby! I was truly not thinking. I also read that it was safe to use rocks from outside, but now I’m not sure? Can anyone help
    +1
    Unlike
  • Saturn:
    @Paige1warren, Since you took it out straight away it should be ok but I'd still be doing a big water change. When I was a teen, I took some rocks from outside, washed them (probably in soap) and put them in my turtle tank. He died a week or so later and I've never used any rocks from the garden since because you just don't know what's been on them, such as pesticide and I suppose the mineral composition of the rock comes in to play too. Letting them dry for a few days before adding them also would have helped so you should be fine
    +1
    Unlike
  • Paige1warren:
    @Saturn, okay, thank you so much! Firstly, I’m so sorry about your turtle :(. My lotl seems to be fine, I haven’t noticed any changes or signs of destress. I do 25% weekly water changes (I have a fully cycled tank), but I will do a 50% change today. I hope that everything turns out fine. Axies are pretty good at showing they are not well or stressed so I’m hoping that I got the rocks out fast enough. Thank you again!
    +1
    Unlike
  • Saturn:
    @Paige1warren, Thanks hun and you're absolutely right, axolotls are so sensitive it would've showed signs. Glad your lotl is ok :)
    +1
    Unlike
  • Pookisoo:
    Hii my tiger salamander has a black bump on his head, its looks like a little spot but its forming into a bump and its like making a hole..? You can tell i need help , please help😁
    +1
    Unlike
  • Pookisoo:
    Do i just give him a salt bath?
    +1
    Unlike
  • madcaplaughs:
    You need to take him to the vet if there's a hole that's forming in his head.
    +1
    Unlike
  • Tanker:
    New to site. Have a Golden Albino and a Black Melanoid
    +1
    Unlike
  • Tanker:
    Tank size 40 gal. 2 filter pumps /substrate roughed ceramic easy to clean / feed each one night crawler every 3 rd day
    +1
    Unlike
  • Murk:
    Ceramic substrate? That sounds interesting. What should I imagine by that?
    +1
    Unlike
    Murk: Ceramic substrate? That sounds interesting. What should I imagine by that? +1
    Top