Caudata.org: Newts and Salamanders Portal

Register a free account today to become a member! Once signed in, you'll be able to participate on this site by adding your own topics and posts, as well as connect with other members through your own private inbox!
Did you know that registered users see fewer ads? Register today!

Chytrid study

lexmiller

New member
Joined
Nov 13, 2009
Messages
131
Reaction score
2
Location
NorCal
I am going into the field to do some herping in Novemember and would like to take some samples from the various herps I find. I know how to swab a herp for chytrid but I am not sure what to store the swabs in (fluid or dry). I was thinking of looking at the samples myself or sending them to the Museum of Vertebrate Zoology at Berkley.

What are your opinions on this?

Before we get into the nasty posts whole "contamination" or that I'm going to be "spreading" the Bd, let me assure you that I am taking all the safety precautions (gloves, bleach solutions, etc...). Let's get past that and just give me your examples of preserving samples and what not. Thanks.

I could do an internet search, but I value the opinions of the experts on this board much more than some random webber.
 

Jennewt

Administrator
Staff member
Joined
May 27, 2005
Messages
12,431
Reaction score
100
Location
USA
If the museum is willing to examine them for you, I'd ask them for advice on sample storage.

Is it really possible to test for chytrid by visual means? I've always heard about it being tested by PCR. For that, you need to store the swabs in alcohol. If you don't have access to a laboratory, the best source of non-denatured ethyl alcohol is a product called Everclear (drinkable high-percentage ethanol).
 

daedae

New member
Joined
Mar 21, 2012
Messages
52
Reaction score
2
Location
Lafayette, Louisiana
For how long are you wanting to preserve the samples? According to the San Diego Zoo, and many other sources, freezing will do just fine. Amphibian Ark recommends storing in a 70% EtOH solution. When I have sent samples from when i kept dendros, I would send them in dry, and not during extreme weather highs.
 
General chit-chat
Help Users
  • No one is chatting at the moment.
  • Shane_Yogurt:
    its around 3 small pieces in the tank. Since i've just moved homes, my axolotl is still at my old house. Yesterday i fed him bloodworms and he missed a few. I couldnt get them out without a turkey baster and decided to let them sit because i was gonna move him to the house tomorrow. But now its late and I dont have a car and my dad wont drive me. Will he be fine?
    +1
    Unlike
  • melon:
    I would think so i would just try to get them out tomorrow
    +1
    Unlike
  • Shane_Yogurt:
    yeah, im heading over tomorrow morning to move him to this house and feed him. Thanks for the help!
    +1
    Unlike
  • Shane_Yogurt:
    So my axolotl tank cycle just crashed and while i was in the middle of a water change my bucket overflowed and spilled water all of the ground in my brand new home. This is going super well 👍
    +2
    Unlike
  • the:
    ooff
    +1
    Unlike
  • the:
    good luck recycling the tank!
    +1
    Unlike
  • Roach:
    do the classifieds still exist?
    +1
    Unlike
  • Roach:
    nevermind! off my game tonight
    +1
    Unlike
  • Shane_Yogurt:
    Im so frustrated right now. My axolotl WONT eat and my tank still isnt looking too good. Some extra stress i needed.
    +1
    Unlike
  • John:
    Sorry to hear that Shane. Did you post about it?
    +1
    Unlike
  • Shane_Yogurt:
    No, I havent. Im not really sure why he wont eat. Hes in a 1 gallon tub and still a juvenile. When i offer food he swims away from it. Does he need some extra time? or is this something I should be worried about.
    +1
    Unlike
  • JulMl:
    Hello everyone! I’m new in this world and i need some advices please! I have 2 axolotl babies and currently the water from the tank is from bottled water ( all parameters are good) but i want to change 50% of the water with city tap water. My question is how to change it? Do i need to get axis out, do the change, add the prime, wait (how much?) until its dechlorinated or i can add the tap water directly into the tank with axis in it, and add the prime conditioner? Thank you!!
    +1
    Unlike
  • Asmold1:
    1. You dont need to take them out of the tank to change the water as long as you pour it in slow as to not rattle them around too much
    +1
    Unlike
  • Asmold1:
    2. add the prime to your tap water, for most conditioners the consensus is 5 minutes of waiting time
    +1
    Unlike
  • Asmold1:
    3.After 5 minutes it should be safe to add
    +1
    Unlike
  • JulMl:
    Thank you so much !!
    +1
    Unlike
  • Asmold1:
    I private messaged you a bit clearer instructions just in case
    +2
    Unlike
  • tammyaxie:
    Where can I get blackworms?
    +1
    Unlike
  • John:
    Ebay or Eastern Aquatics
    +1
    Unlike
  • noahc808:
    Does anyone know someone who can ship axolotls to hawaii? I recently did a water change and my axolotl died and there are no axos for sale right now.
    +1
    Unlike
  • Chat Bot:
    GadgetInspector has left the room.
    +1
    Unlike
  • Chat Bot:
    GadgetInspector has joined the room.
    +1
    Unlike
  • Chat Bot:
    Captive Bread has left the room.
    +1
    Unlike
  • Chat Bot:
    Captive Bread has joined the room.
    +1
    Unlike
  • Chat Bot:
    Captive Bread has left the room.
    +1
    Unlike
    Chat Bot: Captive Bread has left the room. +1
    Top