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Photos of jeffersons

P

paris

Guest
i got a break in school-test is moved to next week so i decided to post some pics i was promising. these arent too outstanding cause i was limited by light, plus these and the giants are pretty shiny sals and i have to try hard to reduce glare-i thinking one of those light umbrellas are probably in my future too. these were raised from an egg mass collected by a teacher and are about 4 years old now.by next semester i will be able for sure to id them as either true jeffersons or as jefferson complex ones-i will be taking genetics lab and the teacher already said i can modify the course so i can do sal DNA and not plants and dog testicles
sick.gif

i am hoping that i can get enough dna from a toe clipping and some amplification techniques.

ok here are some head shots
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14030.jpg


the pattern is hard to see but its a slate-ish gray body with light bluish snowflake marks on it. here is one of the only full body shots that came out ok.
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and finally one obligatory ultra close head shot
14032.jpg
 
K

kaysie

Guest
Paris, is the snowflake markings more concentrated on the flanks and less on the back (as mine are)?
 

megan

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Paris,
I am interested to know how this project went. Is it still on-going? Did you find out what these individuals were - species or hb?
 
L

liz

Guest
paris, if you can't extract enough DNA from a toe clip, you can also take about a millimeter off the end of the tail. once you chop it up and do the extractions, you should have more than enough DNA for you project.
 
P

paris

Guest
the project will be next semester(spring) - i was wondering if shed skin is a viable option -the teacher suggested it.

liz,
is that toe/tail tip suggestion including PCR, or with out it?
 
L

liz

Guest
i don't know about DNA from shed skin in salamanders... i would guess you wouldn't get very much but i don't know for sure.

i have done some genetic work with newt tissues (Taricha, and taking a bit of the tail worked quite well. it didn't make them bleed and it regenerated quickly. we then chop up the tissue and use a kit to extract the DNA from it. then use those extracts for PCR. its not uncommon to have so much DNA in the extract that it has to be diluted before using it for PCR.
 
B

benjamin

Guest
Couldn't you start a cell culture from a very small number of cells and then test the DNA, so nobody has to lose a toe or the tip of their tale.
 
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  • Shane_Yogurt:
    its around 3 small pieces in the tank. Since i've just moved homes, my axolotl is still at my old house. Yesterday i fed him bloodworms and he missed a few. I couldnt get them out without a turkey baster and decided to let them sit because i was gonna move him to the house tomorrow. But now its late and I dont have a car and my dad wont drive me. Will he be fine?
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  • melon:
    I would think so i would just try to get them out tomorrow
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  • Shane_Yogurt:
    yeah, im heading over tomorrow morning to move him to this house and feed him. Thanks for the help!
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  • Shane_Yogurt:
    So my axolotl tank cycle just crashed and while i was in the middle of a water change my bucket overflowed and spilled water all of the ground in my brand new home. This is going super well 👍
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    ooff
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    good luck recycling the tank!
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    nevermind! off my game tonight
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    Im so frustrated right now. My axolotl WONT eat and my tank still isnt looking too good. Some extra stress i needed.
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    Sorry to hear that Shane. Did you post about it?
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    No, I havent. Im not really sure why he wont eat. Hes in a 1 gallon tub and still a juvenile. When i offer food he swims away from it. Does he need some extra time? or is this something I should be worried about.
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    Hello everyone! I’m new in this world and i need some advices please! I have 2 axolotl babies and currently the water from the tank is from bottled water ( all parameters are good) but i want to change 50% of the water with city tap water. My question is how to change it? Do i need to get axis out, do the change, add the prime, wait (how much?) until its dechlorinated or i can add the tap water directly into the tank with axis in it, and add the prime conditioner? Thank you!!
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    1. You dont need to take them out of the tank to change the water as long as you pour it in slow as to not rattle them around too much
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    2. add the prime to your tap water, for most conditioners the consensus is 5 minutes of waiting time
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    3.After 5 minutes it should be safe to add
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    Thank you so much !!
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    I private messaged you a bit clearer instructions just in case
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    Where can I get blackworms?
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    Ebay or Eastern Aquatics
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