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Chytrid study

lexmiller

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I am going into the field to do some herping in Novemember and would like to take some samples from the various herps I find. I know how to swab a herp for chytrid but I am not sure what to store the swabs in (fluid or dry). I was thinking of looking at the samples myself or sending them to the Museum of Vertebrate Zoology at Berkley.

What are your opinions on this?

Before we get into the nasty posts whole "contamination" or that I'm going to be "spreading" the Bd, let me assure you that I am taking all the safety precautions (gloves, bleach solutions, etc...). Let's get past that and just give me your examples of preserving samples and what not. Thanks.

I could do an internet search, but I value the opinions of the experts on this board much more than some random webber.
 

Jennewt

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If the museum is willing to examine them for you, I'd ask them for advice on sample storage.

Is it really possible to test for chytrid by visual means? I've always heard about it being tested by PCR. For that, you need to store the swabs in alcohol. If you don't have access to a laboratory, the best source of non-denatured ethyl alcohol is a product called Everclear (drinkable high-percentage ethanol).
 

daedae

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For how long are you wanting to preserve the samples? According to the San Diego Zoo, and many other sources, freezing will do just fine. Amphibian Ark recommends storing in a 70% EtOH solution. When I have sent samples from when i kept dendros, I would send them in dry, and not during extreme weather highs.
 
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  • blubford:
    my idea was to have two one in the tank and one freezing and by the time the first melts i switch it out with the one freezing and repeat
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  • blubford:
    and for the land i think they can just go into the water to cool off
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  • axolotl nerd:
    yeah i think that sounds like a good plan :)
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  • NeilP:
    Hello
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  • NeilP:
    I have two axolotls (one boy and one girl) and I recently changed them to feeding every other day. Since then, the female has lost two gils and then red marks appeared on two of her legs Which my local aquatic centre said looked like bite marks rather than red leg) and this morning, the female's front left leg is having on by a thread. I was going to the by a divider for my tank to stop them mating but now it seems I need to buy it asap to stop the male eating the female. I have a 125 ltr tank so space should not be an issue, but does anyone have any advice and will the female need a salt bath or anything else?
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  • the:
    she should be ok as axolotls can regenerate limbs, the only this i would say is to mabey feed the male more as this type of behavuor is uslay down to hunger.
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  • Shane_Yogurt:
    hey, anyone know how long bloodworms can stay in an axolotl tank before they begin to rot and cause an ammonia spike?
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  • melon:
    I think it is always best to get them out asap but probably two days or so.
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  • Shane_Yogurt:
    its around 3 small pieces in the tank. Since i've just moved homes, my axolotl is still at my old house. Yesterday i fed him bloodworms and he missed a few. I couldnt get them out without a turkey baster and decided to let them sit because i was gonna move him to the house tomorrow. But now its late and I dont have a car and my dad wont drive me. Will he be fine?
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  • melon:
    I would think so i would just try to get them out tomorrow
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  • Shane_Yogurt:
    yeah, im heading over tomorrow morning to move him to this house and feed him. Thanks for the help!
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  • Shane_Yogurt:
    So my axolotl tank cycle just crashed and while i was in the middle of a water change my bucket overflowed and spilled water all of the ground in my brand new home. This is going super well 👍
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  • the:
    ooff
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  • the:
    good luck recycling the tank!
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  • Roach:
    do the classifieds still exist?
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  • Roach:
    nevermind! off my game tonight
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  • Shane_Yogurt:
    Im so frustrated right now. My axolotl WONT eat and my tank still isnt looking too good. Some extra stress i needed.
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  • John:
    Sorry to hear that Shane. Did you post about it?
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  • Shane_Yogurt:
    No, I havent. Im not really sure why he wont eat. Hes in a 1 gallon tub and still a juvenile. When i offer food he swims away from it. Does he need some extra time? or is this something I should be worried about.
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  • JulMl:
    Hello everyone! I’m new in this world and i need some advices please! I have 2 axolotl babies and currently the water from the tank is from bottled water ( all parameters are good) but i want to change 50% of the water with city tap water. My question is how to change it? Do i need to get axis out, do the change, add the prime, wait (how much?) until its dechlorinated or i can add the tap water directly into the tank with axis in it, and add the prime conditioner? Thank you!!
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  • Asmold1:
    1. You dont need to take them out of the tank to change the water as long as you pour it in slow as to not rattle them around too much
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  • Asmold1:
    2. add the prime to your tap water, for most conditioners the consensus is 5 minutes of waiting time
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  • Asmold1:
    3.After 5 minutes it should be safe to add
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  • JulMl:
    Thank you so much !!
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  • Asmold1:
    I private messaged you a bit clearer instructions just in case
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    Asmold1: I private messaged you a bit clearer instructions just in case +2
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