Kanamycin/Kanaplex for infection?

[vak14]

Has anyone used Kanamycin for a suspected bacterial infection? I posted earlier about my axolotl suffering from bloat that I believed was caused from ammonia/nitrite poisoning. I tubbed her and dosed her with some methylene blue and she improved initially, but then seemed to get a bit worse. I am planning on moving her to my basement/fridging so she can be kept at a cooler temp and potentially dosing with kanamycin for possible septicemia/bacterial infection. I was wondering if anyone else has tried this or what dosage would be recommended. I called Seachem (who produces Kanaplex) and they recommend roughly 125 mg per 5 gallons, although their product is not entirely Kanamycin and is not officially approved for use in axolotls. I have some kanamycin I would like to try but was unsure if I should use the same dosage. Is it even possible to overdose her on it, or are there any negative side effects?

 

[wolfen]

try using holtfreters solution first go to Axolotls - Requirements & Water Conditions in Captivity for details on chemicals required.

 

[vak14]

try using holtfreters solution first go to Axolotls - Requirements & Water Conditions in Captivity for details on chemicals required.

Hello my friend- I can’t find all those chemicals in a timely fashion. Also, i’m hesitant to use it since it would be a change to her husbandry I have not done before. Also, do you have any recommendations on where to find those chemicals?

 

[wolfen]

most of the chemicals can be brought online johns solution is the easiest to make up, NaCl is marine salt so pet shop (don't use salt for human consumption such as table salt as it contains iodine), MgSO4.7H2O is epsom salt so a chemist/pharmacy, NaHCO3 is bicarbonate of soda (baking soda). so any shop that sells cooking ingredients will have it. because axolotls are from slight brackish high mineral environment they require salts and minerals to stay healthy.

 

[vak14]

most of the chemicals can be brought online johns solution is the easiest to make up, NaCl is marine salt so pet shop (don't use salt for human consumption such as table salt as it contains iodine), MgSO4.7H2O is epsom salt so a chemist/pharmacy, NaHCO3 is bicarbonate of soda (baking soda). so any shop that sells cooking ingredients will have it. because axolotls are from slight brackish high mineral environment they require salts and minerals to stay healthy.

Thank you. what would be the dosage per gallon of johns solution? also if i am unable to get these things until tomorrow is it still advisable to fridge/reduce temp?

 

[vak14]

most of the chemicals can be brought online johns solution is the easiest to make up, NaCl is marine salt so pet shop (don't use salt for human consumption such as table salt as it contains iodine), MgSO4.7H2O is epsom salt so a chemist/pharmacy, NaHCO3 is bicarbonate of soda (baking soda). so any shop that sells cooking ingredients will have it. because axolotls are from slight brackish high mineral environment they require salts and minerals to stay healthy.

also, should i add both johns and kanamycin to the water, since johns isn’t technically medication? and for how long should i use johns before i switch to kanamycin?

 

[wolfen]

for johns solution it is 1g per litre salt, 0.1g per litre epsom salts, 0.1g per litre bicarbonate of soda, keep her in cool fresh dechlorinated water provided the water is between 15°c/59°f and 18°c/65°f she will be fine, you can keep her cooler then that if you can but I would refrain from fridging her until there there is no other options due to the inherent risks of fridging.

 

[vak14]

for johns solution it is 1g per litre salt, 0.1g per litre epsom salts, 0.1g per litre bicarbonate of soda, keep her in cool fresh dechlorinated water provided the water is between 15°c/59°f and 18°c/65°f she will be fine, you can keep her cooler then that if you can but I would refrain from fridging her until there there is no other options due to the inherent risks of fridging.

okay, i moved her to my basement which is 55 F for now. how long should i wait for the johns to take effect before i move on to antibiotics if there is no improvement?

 

[wolfen]

okay, i moved her to my basement which is 55 F for now. how long should i wait for the johns to take effect before i move on to antibiotics if there is no improvement?

about a week should be long enough.

 

[vak14]

about a week should be long enough.

but if it is an infection won't it be too late by then? is it possible to use both john's solution and kanamycin just in case? i have half doses kanamycin (.0125 g/gal) which i measured out at my university lab just in case. i figured half doses over full ones because it is easier to add more later on if I wanted to. since this has already been going on for about a week I don't want to delay anything and would like to try everything at the same time. I tried calling every vet within an 2 hours of my house but everyone turned me away or didn't know about axolotls.

 

[wolfen]

but if it is an infection won't it be too late by then? is it possible to use both john's solution and kanamycin just in case? i have half doses kanamycin (.0125 g/gal) which i measured out at my university lab just in case. i figured half doses over full ones because it is easier to add more later on if I wanted to. since this has already been going on for about a week I don't want to delay anything and would like to try everything at the same time. I tried calling every vet within an 2 hours of my house but everyone turned me away or didn't know about axolotls.

if you want to use anti-biotics at any time with johns solution (modified holtfreters solution) you can.

 

[vak14]

if you want to use anti-biotics at any time with johns solution (modified holtfreters solution) you can.

thank you. I will start with antibiotics tonight and work on making john's solution tomorrow or the next day when I can get into the lab

 

[vak14]

if you want to use anti-biotics at any time with johns solution (modified holtfreters solution) you can.

one more question- should i try to feed her at all during this time? she hasn't eaten at all in about 2 1/2, 3 weeks, but I haven't offered her anything in the last several days at all so I don't know if she would take food. I have canadian nightcrawlers and frozen bloodworms on hand but I could look for something else as well

 

[wolfen]

one more question- should i try to feed her at all during this time? she hasn't eaten at all in about 2 1/2, 3 weeks, but I haven't offered her anything in the last several days at all so I don't know if she would take food. I have canadian nightcrawlers and frozen bloodworms on hand but I could look for something else as well

if she wants to eat let her.

 

[vak14]

if she wants to eat let her.

Hello friend, wanted to thank you for all your help and update that my axie seems on the mend with the kanamycin/johns blend. Pinker gills, abdomen a little less bloated every day. I have been using Johns at full strength (1g NaCl, .1g epsom, .1g bicarb of soda per L) and full dose kanamycin. I had to convert dosages to gallons since she is being kept in about 1 gal of water. She is still somewhat lethargic but also still in my basement so her temps are ranging between 52 F and 56 F. Offered food but wasn’t interested. Any advice on when/how I could move her back to her aquarium, or even back out of the basement while still being tubbed and treated?

 

[wolfen]

Hello friend, wanted to thank you for all your help and update that my axie seems on the mend with the kanamycin/johns blend. Pinker gills, abdomen a little less bloated every day. I have been using Johns at full strength (1g NaCl, .1g epsom, .1g bicarb of soda per L) and full dose kanamycin. I had to convert dosages to gallons since she is being kept in about 1 gal of water. She is still somewhat lethargic but also still in my basement so her temps are ranging between 52 F and 56 F. Offered food but wasn’t interested. Any advice on when/how I could move her back to her aquarium, or even back out of the basement while still being tubbed and treated?

continue using johns solution as part of the normal water change routine, provided the water parameters are good and the temperature is good float the container in the tank to allow the temperature to equalise and allow her to go back into the tank. make sure the tank water contains the same level of johns solution. give her food at night and leave it till morning before doing any cleaning.

 

[vak14]

continue using johns solution as part of the normal water change routine, provided the water parameters are good and the temperature is good float the container in the tank to allow the temperature to equalise and allow her to go back into the tank. make sure the tank water contains the same level of johns solution. give her food at night and leave it till morning before doing any cleaning.

Hi friend. Wanted to update. Axie is still doing okay. Eating some bloodworms (small amount almost every day) since last Sunday but now my worry is still no poop. Bloat originally decreased but now seems stable/slightly more than last Sunday I am assuming due to the eating/not pooping. Anything I can try or wait it out? Otherwise gills a lovely pink, no floating, no gasping for air. Changing tub water every 24 hrs.

 

[wolfen]

you can increase the salt content up to 3g per litre to reduce any more bloating due to water absorption.
make sure to keep the water cold and oxygenated.
if she's a adult her feeding can be reduced to every other day.
try feeding her something more digestible every other feeding such as frozen brineshrimp.
if she young/juvenile keep feed her as you are.
also the amount of epsom salts can be increased to 0.2g per litre this should have a slight laxative effect.
right now it's just a waiting game

 

[jennerosa]

Increase fiber in the diet: Consider adding high-fiber foods to Axi's diet, such as blanched peas or small pieces of cooked vegetables like spinach or lettuce. This can promote healthy digestion and encourage bowel emptying. Adjust the number of feedings: Take care not to overfeed Aksi, as excessive eating can lead to digestive problems. And while fighting digestion, I suggest playing $1 minimum deposit minimum deposit online casino in Canada, with a list of such games already ready - https://casinosanalyzer.ca/lоw-deposits/1-dollar-deposit-casino all that's left to do is get together and rip the score) Stick to small, appropriate portions and watch her reaction. Provide gentle stimulation: You can gently massage Axi's belly with clean hands or a soft cloth. This can help stimulate the digestive system and promote bowel emptying. Be careful not to apply too much pressure or cause discomfort. Monitor water parameters: Make sure water quality parameters such as ammonia, nitrite, nitrate and pH are within normal limits. Poor water quality can affect the health and digestion of axolotls. It is important to change the water regularly and monitor its condition.