determining the sex of amystoma in the larval stage

RG

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Hello, a week ago I asked whether a method is already being used for sex determination of Ambystoma salamanders in the larval stage. I am not receiving a response to this. I am now assuming that there is no interest in this or that a method already exists. Kind regards, Ruud.
 
because the larva are still growing (including axolotls up to adult size) it is difficult to use the normal clues towards what their sex is ie.. enlarged genitalia, body length, tail length, torso shape etc..
 
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